Variation in adhesion of Streptococcus mutans and Porphyromonas gingivalis in saliva-derived biofilms on raw materials of orthodontic brackets
박소현, Kim Kyung-Sun, 조소하, 정동화, 안석준,
소속 상세정보
박소현 ( Park So-Hyun ) - Dankook University Jukjeon Dental Hospital Department of Orthodontics
( Kim Kyung-Sun ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
조소하 ( Cho So-Ha ) - Seoul National University School of Dentistry Dental Research Institute
정동화 ( Chung Dong-Hwa ) - Dankook University Jukjeon Dental Hospital Department of Orthodontics
안석준 ( Ahn Sug-Joon ) - Seoul National University School of Dentistry Department of Orthodontics
Abstract
Objective: To evaluate differences in the adhesion levels of the most common oral pathogens, Streptococcus mutans and Porphyromonas gingivalis , in human saliva-derived microcosm biofilms with respect to time and raw materials of orthodontic brackets.
Methods: The samples were classified into three groups of bracket materials: 1) monocrystalline alumina ceramic (CR), 2) stainless steel metal (SS), and 3) polycarbonate plastic (PL), and a hydroxyapatite (HA) group was used to mimic the enamel surface. Saliva was collected from a healthy donor, and saliva-derived biofilms were grown on each sample. A real-time polymerase chain reaction was performed to quantitatively evaluate differences in the attachment levels of total bacteria, S. mutans and P. gingivalis at days 1 and 4.
Results: Adhesion of S. mutans and P. gingivalis to CR and HA was higher than the other bracket materials (SS = PL < CR = HA). Total bacteria demonstrated higher adhesion to HA than to bracket materials, but no significant differences in adhesion were observed among the bracket materials (CR = SS = PL < HA). From days 1 to 4, the adhesion of P. gingivalis decreased, while that of S. mutans and total bacteria increased, regardless of material type.
Conclusions: The higher adhesion of oral pathogens, such as S. mutans and P. gingivalis to CR suggests that the use of CR brackets possibly facilitates gingival inflammation and enamel decalcification during orthodontic treatment.
키워드
Bracket; Microbiology; Multispecies biofilms; Oral pathogen
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